Xanthophyll was slightly more polar due to its -OH group, so it came after Beta carotene. Chlorophyll a and b stuck to the bottom line because they were more polar than the carotenoids due to their shorter hydrocarbon chain and Magnesium atom.
Is chlorophyll a more polar?
The distinctions between the chlorophylls, which are more polar than β-carotene is slight: chlorophyll a has a methyl group (Y=CH3) in a position where chlorophyll b has an aldehyde (Y=CHO). This makes chlorophyll b slightly more polar than chlorophyll a.
Which plant pigment is most polar?
Chlorophyll b
Chlorophyll b is a more polar (water-loving) pigment than the other pigments found in spinach extracts and is therefore more strongly attracted to the polar surface of the paper than to the nonpolar solvent.
Which are more soluble in the chromatography solvent xanthophylls or chlorophyll A?
The orange colored band, made of the pigment called carotenoids. is the most soluble in alcohol, so it traveled the farthest. The yellow xanthophylls are the next most soluble, followed by the blue-green chlorophyll A.
What is the most polar xanthophyll?
From this, we can deduce that carotenes are the least polar pigments (no polar groups), and xanthophylls are the most polar (two alcohol groups, one at each end of the molecule). Therefore, pigments 1 and 2 are likely to be carotenes, and pigment 4 is likely to be a xanthophyll.
Why does chlorophyll b travel the least?
Chlorophyll b traveled least because it was the least soluble in the solvent. This lab showed that there was much more green pigments (cholorophyll a and b) than yellow pigments because the green bands were the darkest and thickest on the paper. That is why spinach leaves are green.
Is chlorophyll a more polar than lutein?
Chlorophyll b is a more polar (water-loving) pigment than the other pigments found in spinach extracts and is therefore more strongly attracted to the polar surface of the paper than to the nonpolar solvent. Lutein and zeaxanthin belong to the class of carotenoids known as xanthophylls and both contain hydroxyl groups.
Is chlorophyll a or Pheophytin a more polar?
Both chlorophylls contain C-O and C-N bonds, which are polar, and also contain magnesium bonded to nitrogen, which is such a polar bond it is almost ionic. Both chlorophylls are much more polar than -carotene. Pheophytin a is chlorophyll a without the Mg-ion.
Why does Xanthophyll travel the least?
Xanthophyll contains oxygen and does not travel quite as far with the solvent because it is less soluble the beta carotene and forms hydrogen bonds with the paper. Chlorophylls are bound more tightly to the paper than the other two, so they travel the shortest distance.
Which is the least polar chlorophyll or xanthophyll?
From this, we can deduce that carotenes are the least polar pigments (no polar groups), and xanthophylls are the most polar (two alcohol groups, one at each end of the molecule). Pigment 3 is likely to be chlorophyll, since it is more polar than carotenes but less polar than xanthophylls. Also to know is, is chlorophyll polar or nonpolar?
Which is more polar carotene or pheophytin?
Both pheophytin and chlorophyll are more polar than carotene due to polar functional group such as ketone and ester group in their structure.
Which is more polar lutein or zeaxanthin?
Lutein and zeaxanthin belong to the class of carotenoids known as xanthophylls and both contain hydroxyl groups. This makes them more polar than carotenoids, such as beta-carotene and lycopene, which do not contain oxygen.